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pax7 ab 528428 myod mouse igg2b  (Developmental Studies Hybridoma Bank)


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    Developmental Studies Hybridoma Bank pax7 ab 528428 myod mouse igg2b
    Pax7 Ab 528428 Myod Mouse Igg2b, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 99/100, based on 254 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+pax7+ab/anti-Pax7/pm41462267-289-8-7
    Average 99 stars, based on 254 article reviews
    pax7 ab 528428 myod mouse igg2b - by Bioz Stars, 2026-09
    99/100 stars

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    Incubation:

    Article Title: Full-length Dysferlin Transfer by the Hyperactive Sleeping Beauty Transposase Restores Dysferlin-deficient Muscle
    Article Snippet: .. The sections were incubated overnight at 4 °C with mouse-anti-Pax7 ab (DSHB, stock as above, working dilution 1:5) and Romeo ab (1:50). ..



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    Figure 6. Impaired mitochondrial fission causes regenerative failure in old satellite cells (A) Commonly altered pathways detected in young versus old mouse 1DPI-SCs and in Drp1WT versus Drp1DPax7ER datasets. Commonly altered DE genes were analyzed in g:Profiler. The top gene ontology (GO), Reactome, and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways are shown (left). Enrichment map of common pathway clusters enriched in old or Drp1DPax7ER 1DPI-SCs. Orange nodes represent pathways downregulated in old versus young 1DPI-SCs (FDR < 0.25); blue nodes represent pathways downregulated in Drp1DPax7ER versus Drp1WT 1DPI-SCs. (B) Representative IF images of <t>PAX7,</t> DRP1, and DAPI staining (top), and quantification of DRP1 signal of young and old 3-DPI TA muscles (bottom). (C) Representative IF images of total and active DRP1 (pDRP, phosphorylated DRP1) staining (top) and quantification of DRP1 (lower left) and pDRP (lower right) signals in 3DPI-SCs from young or old mice. (D) Representative images of MitoTracker (top, upper panel) and 3D-reconstructed mitochondrial network (top, lower panel) in 3DPI-SCs from young or old mice and quantification of average mitochondrial volume in each cell (bottom). Reconstructed mitochondria were colored based on their sphericity (0–1). (E) Schematics of transplantation of satellite cells from old mice after ex vivo DRP1 or control lentiviral vector transduction (left) into preinjured muscles of immunodeficient mice. Representative images are shown of Dil and DAPI (middle) and quantification (right) of Dil+ cells per area. Data were analyzed at 4 DPI post-transplantation. The values presented are relative to the level of control (LV-control-treated group). Data represent n = 3–4 mice and are mean ± SD. p values were determined by a two-tailed unpaired t test. IntDen, integrated density. Scale bars: 5 mm in (B), 3 mm in (C) and (D), and 25 mm in (E). See also Figure S6 and Table S3.
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    Image Search Results


    Figure 6. Impaired mitochondrial fission causes regenerative failure in old satellite cells (A) Commonly altered pathways detected in young versus old mouse 1DPI-SCs and in Drp1WT versus Drp1DPax7ER datasets. Commonly altered DE genes were analyzed in g:Profiler. The top gene ontology (GO), Reactome, and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways are shown (left). Enrichment map of common pathway clusters enriched in old or Drp1DPax7ER 1DPI-SCs. Orange nodes represent pathways downregulated in old versus young 1DPI-SCs (FDR < 0.25); blue nodes represent pathways downregulated in Drp1DPax7ER versus Drp1WT 1DPI-SCs. (B) Representative IF images of PAX7, DRP1, and DAPI staining (top), and quantification of DRP1 signal of young and old 3-DPI TA muscles (bottom). (C) Representative IF images of total and active DRP1 (pDRP, phosphorylated DRP1) staining (top) and quantification of DRP1 (lower left) and pDRP (lower right) signals in 3DPI-SCs from young or old mice. (D) Representative images of MitoTracker (top, upper panel) and 3D-reconstructed mitochondrial network (top, lower panel) in 3DPI-SCs from young or old mice and quantification of average mitochondrial volume in each cell (bottom). Reconstructed mitochondria were colored based on their sphericity (0–1). (E) Schematics of transplantation of satellite cells from old mice after ex vivo DRP1 or control lentiviral vector transduction (left) into preinjured muscles of immunodeficient mice. Representative images are shown of Dil and DAPI (middle) and quantification (right) of Dil+ cells per area. Data were analyzed at 4 DPI post-transplantation. The values presented are relative to the level of control (LV-control-treated group). Data represent n = 3–4 mice and are mean ± SD. p values were determined by a two-tailed unpaired t test. IntDen, integrated density. Scale bars: 5 mm in (B), 3 mm in (C) and (D), and 25 mm in (E). See also Figure S6 and Table S3.

    Journal: Cell stem cell

    Article Title: Mitochondrial dynamics maintain muscle stem cell regenerative competence throughout adult life by regulating metabolism and mitophagy.

    doi: 10.1016/j.stem.2022.07.009

    Figure Lengend Snippet: Figure 6. Impaired mitochondrial fission causes regenerative failure in old satellite cells (A) Commonly altered pathways detected in young versus old mouse 1DPI-SCs and in Drp1WT versus Drp1DPax7ER datasets. Commonly altered DE genes were analyzed in g:Profiler. The top gene ontology (GO), Reactome, and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways are shown (left). Enrichment map of common pathway clusters enriched in old or Drp1DPax7ER 1DPI-SCs. Orange nodes represent pathways downregulated in old versus young 1DPI-SCs (FDR < 0.25); blue nodes represent pathways downregulated in Drp1DPax7ER versus Drp1WT 1DPI-SCs. (B) Representative IF images of PAX7, DRP1, and DAPI staining (top), and quantification of DRP1 signal of young and old 3-DPI TA muscles (bottom). (C) Representative IF images of total and active DRP1 (pDRP, phosphorylated DRP1) staining (top) and quantification of DRP1 (lower left) and pDRP (lower right) signals in 3DPI-SCs from young or old mice. (D) Representative images of MitoTracker (top, upper panel) and 3D-reconstructed mitochondrial network (top, lower panel) in 3DPI-SCs from young or old mice and quantification of average mitochondrial volume in each cell (bottom). Reconstructed mitochondria were colored based on their sphericity (0–1). (E) Schematics of transplantation of satellite cells from old mice after ex vivo DRP1 or control lentiviral vector transduction (left) into preinjured muscles of immunodeficient mice. Representative images are shown of Dil and DAPI (middle) and quantification (right) of Dil+ cells per area. Data were analyzed at 4 DPI post-transplantation. The values presented are relative to the level of control (LV-control-treated group). Data represent n = 3–4 mice and are mean ± SD. p values were determined by a two-tailed unpaired t test. IntDen, integrated density. Scale bars: 5 mm in (B), 3 mm in (C) and (D), and 25 mm in (E). See also Figure S6 and Table S3.

    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti- Citrate synthetase abcam Cat# ab 96600; RRID:AB_10678258 Mouse monoclonal anti- TOM20 abcam Cat# ab56783;RRID: AB_945896 Mouse monoclonal anti- eMHC DHSB Cat# F1.652; RRID:AB_528358 Chicken anti- GFP AVES Labs Cat# GFP-1020; RRID:AB_10000240 Rabbit polyclonal anti- KI67 abcam Cat# ab15580; RRID:AB_443209 Rabbit polyclonal anti- Laminin Novus Biological Cat# NB300-144AF647; RRID:AB_2891039 Rabbit polyclonal anti- MYOD Santa Cruz Cat# sc-760; RRID:AB_2148870 Rabbit polyclonal anti- Myogenin Santa Cruz Cat# sc-576;RRID: AB_2148908 Mouse monoclonal anti- PAX7 Santa Cruz Cat# sc-81648; RRID:AB_2159836 Rabbit monoclonal anti- DRP1 Cell Signaling Technology Cat# 8570;RRID: AB_10950498 Rabbit polyclonal anti- phospho-DRP1 (Ser616) Cell Signaling Technology Cat# 3455; RRID: AB_2085352 Mouse monoclonal anti- CD56 (NCAM) Monosan Cat# MON 9006; RRID:AB_419798 Mouse monoclonal anti- dsDNA abcam Cat# ab27156;RRID: AB_470907 Mouse monoclonal anti- LC3 Nanotools Cat# 0231-100; RRID:AB_2722733 Rabbit polyclonal anti- p62/SQSTM1 Merck Cat# P0067; RRID:AB_1841064 Rabbit polyclonal anti- Parkin abcam Cat# ab15494;RRID: AB_301903 Rat monoclonal anti- LAMP1 Santa Cruz Cat# sc-19992; RRID:AB_2134495 Rabbit anti- g-H2AX Cell Signaling Technology Cat# 2577; RRID:AB_2118010 Rabbit polyclonal anti- Cleaved Caspase-3 (Asp175) Cell Signaling Technology Cat# 9661; RRID:AB_2341188 Rat monoclonal anti- BrdU abcam Cat# ab 6326;RRID: AB_305426 Alexa Fluor 488 goat anti mouse Invitrogen Cat# A11017; RRID:AB_2534084 Alexa Fluor 488 goat anti mouse Invitrogen Cat# A21121 RRID:AB_2535764 Alexa Fluor 488 goat anti rabbit Invitrogen Cat# A11008; RRID:AB_143165 Alexa Fluor 568 goat anti mouse Invitrogen Cat# A21124; RRID:AB_2535766 Alexa Fluor 568 goat anti rabbit Invitrogen Cat# A11036; RRID:AB_10563566 (Continued on next page) Cell Stem Cell 29, 1298–1314.e1–e10, September 1, 2022 e1

    Techniques: Staining, Muscles, Transplantation Assay, Ex Vivo, Control, Plasmid Preparation, Transduction, Two Tailed Test